Pembalap Aspar Team, Yoni Hernandez menjadi yang tercepat di sesi latihan resmi pertama MotoGP Mugelo Italia 2016. Ia mencatat waktu 1.54,199. Yoni menjadi pembalap tercepat dengan menggunakan ban intermediate. Berikut ini hasil lengkap latihan resmi pertama.
1. Yonny Hernandez COL Aspar MotoGP Team (Desmosedici GP14.2) 1m 54.199s [Lap 10/10] 326km/h (Top Speed)
2. Scott Redding GBR Octo Pramac Yakhnich (Desmosedici GP15) 1m 54.973s +0.774s [12/12] 335km/h
3. Cal Crutchlow GBR LCR Honda (RC213V) 1m 55.334s +1.135s [12/12] 326km/h
4. Bradley Smith GBR Monster Yamaha Tech 3 (YZR-M1) 1m 55.659s +1.460s [12/12] 330km/h
5. Michele Pirro ITA Ducati Team (Desmosedici GP) 1m 56.411s +2.212s [8/8] 333km/h
6. Maverick Viñales ESP Team Suzuki Ecstar (GSX-RR) 1m 56.966s +2.767s [8/8] 330km/h
7. Eugene Laverty IRL Aspar MotoGP Team (Desmosedici GP14.2) 1m 58.757s +4.558s [10/10] 317km/h
8. Alvaro Bautista ESP Factory Aprilia Gresini (RS-GP) 1m 59.101s +4.902s [6/6] 309km/h
9. Andrea Iannone ITA Ducati Team (Desmosedici GP) 1m 59.808s +5.609s [5/6] 325km/h
10. Tito Rabat ESP Estrella Galicia 0,0 Marc VDS (RC213V)* 2m 00.742s +6.543s [18/18] 305km/h